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KMID : 0376319980100010223
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Dental Journal of CNU
1998 Volume.10 No. 1 p.223 ~ p.230
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Effect of media and buffer on the formation of artificial plaque
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Shin Kyung-Mi
Oh Jong-Suk
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Abstract
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Various kinds of factor affect the formation of dental plaque by Streptococcus mutans in the oral cavity. These factors include the nutrient molecules and chemical agents. The components of media affecting the bacterial replicaton and the formation of artificial plaque were assessed on orthodontic wire by culturing Streptococcus mutans with the following.
1. The bacterial replication and the formation of artificial plaque were most prominent in M17 broth among studied media.
2. The artificial plaque was significantly formed in the media containing 0.5% yeast extract. The bacterial replication and the formation of artificial plaque were significant in the media containing 5% sucrose, while being little in the media containing 20% sucrose
3. Sodium bicarbonate at 100mM or above completely inhibited the bacterial replication and the formation of artificial plaque. The bacterial replication was significant in the media containing 10mM Tris. The formation of artificial plaque was inhibited a little at that concentration, and completely inhibited in the media containing 100mM Tris.
4. The formation of artificial plaque was inhibited in the media containing 100mM sodium phosphate, while the cell concentration was decreased a little compared with the control. The bacterial replication was significant in the media containing 1mM potassium phosphate, while the bacterial replication and the formation of artificial plaque were inhibited at 100mM.
5. The bacterial replication was inhibited in the media containing 100mM MOPS or HEPES, while increased in the media containing 100mM TES. The formation of artificial plaque was inhibited a little respectively.
These results indicate that the formation of artificial plaque is increased in proportion to the bacterial replication, and Tris or sodium phosphate may affect the activity of glucosyltransferase, resulting into attenuating the formation of artificial plaque.
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